DNA

Part:BBa_K4687020:Design

Designed by: Yiming Jiang   Group: iGEM23_HBUT-China   (2023-10-02)


Donor:crtI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1005
    Illegal PstI site found at 483
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1005
    Illegal NheI site found at 870
    Illegal PstI site found at 483
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1005
    Illegal BglII site found at 1248
    Illegal BglII site found at 1611
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1005
    Illegal PstI site found at 483
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1005
    Illegal PstI site found at 483
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

After cleaving the DNA gap by CRISPR-MAD7 nuclease, the donor DNA undergoes homologous recombination repair to achieve the knockout of the targeted gene.


Source

It is derived from a continuous random sequence of 1000bp in length with homology effects at the front and end of the CRISPR-MAD7 nuclease binding to the PAM region.

References